Palate Development: Normal and Abnormal Cellular and by A.A. Moscona and Alberto Monroy (Eds.)

By A.A. Moscona and Alberto Monroy (Eds.)

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Extra resources for Palate Development: Normal and Abnormal Cellular and Molecular Aspects

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19791. Nucleic Acids Res. 7, 2081-2103. , and Shields, E. D. I n “Clinical Dysmorphology of Oral-Facial Structures’’ (M. Melnick, E. D. Shields, and N. J . ), pp. 360-372. Wright, Boston. , and Slavkin, H. C. (1981). Immunogenetics 13, 443-449. Needham, J. (1966). ” Cambridge Univ. Press, London and New York. Noden, D. W. (1975). Deu. Biol. 42, 106-130. Noden, D. W. (1982). I n “Factors and Mechanisms Influencing Bone Growth” (A. D. Dixon and B. G. ), pp. 167-204. Liss, New York. O’Malley, B. , Towle, H.

Images of adjacent stained and digested/stained sections are registered with respect t o one another and the brightness values of one image are subtracted from the other. The resultant difference picture reveals the location of material removed by the specific enzyme digestion, producing a map of the distribution of HA. Using these algorithms we are mapping the distribution of GAGS during development of the palate. We have begun by examining the distribution of HA at two time periods during the last 18 hours of palatal development and immediately after shelf reorientation and adhesion.

1981) are also present a t this time. HA molecules occupy large domains and at physiological concentrations their domains may overlap. The entangled molecules form a dense meshwork (Laurent, 1970). HA may also interact with sulfated proteoglycans and collagen to form gel-fiber networks. These networks exert an osmotic pressure which is in large part produced by the HA. HA has nonideal osmotic behavior, that is, osmotic pressure does not increase directly with HA concentration, but rather more rapidly.

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