Nucleic Acid Transfection by Peter Hahn, Elizabeth Scanlan (auth.), Wolfgang Bielke,

By Peter Hahn, Elizabeth Scanlan (auth.), Wolfgang Bielke, Christoph Erbacher (eds.)

Gene supply into Mammalian Cells: an outline on latest ways hired In Vitro and In Vivo, by way of Peter Hahn and Elizabeth Scanlan
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Strategies for the guidance of artificial Transfection Vectors, via Asier Unciti-Broceta, Matthew N. Bacon, and Mark Bradley
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Cationic Lipids: Molecular Structure/Transfection job Relationships and Interactions with Biomembranes, via Rumiana Koynova and Boris Tenchov
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Hyperbranched Polyamines for Transfection, by means of Wiebke Fischer, Marcelo Calderon, and Rainer Haag
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Carbohydrate Polymers for Nonviral Nucleic Acid supply, by means of Antons Sizovs, Patrick M. McLendon, Sathya Srinivasachari,

and Theresa M. Reineke
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Cationic Liposome–Nucleic Acid Complexes for Gene supply and Silencing: Pathways and Mechanisms for Plasmid DNA and siRNA, via Kai ok. Ewert, Alexandra Zidovska, Ayesha Ahmad, Nathan F. Bouxsein, Heather M. Evans, Christopher S. McAllister, Charles E. Samuel, and Cyrus R. Safinya
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Chemically Programmed Polymers for particular DNA and siRNA Transfection, through Eveline Edith Salcher and Ernst Wagner
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Photochemical Internalization: a brand new device for Gene and Oligonucleotide supply, by way of Kristian Berg, Maria Berstad, Lina Prasmickaite, Anette Weyergang, Pål okay. Selbo, Ida Hedfors, and Anders Høgset
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Visualizing Uptake and Intracellular Trafficking of Gene vendors via Single-Particle monitoring, through N. Ruthardt and C. Bräuchle

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NH2 HO HN DMF NH2 CN HO O NH NHBoc 1) Raney Ni H2 , EtOH Boc 2) Boc2O N HO O N Boc O (4) NH3 O 16 16 R H N Cbz NH NEt3, DCM O 16 16 N NH3 1) H2 , Pd/C DCM:EtOH H N 2) 4, DCC, DCM Cbz 3) CF3COOH R = p-nitrophenyloxy Cbz = benzyloxycarbonyl O 16 16 N H N H2N NH2 O 4 CF3COO DOGS (5) Fig. 2 Cationic Headgroups: Classes and Synthesis Due to the difficulties in handling and purifying the positively-charged reagents, the cationic headgroups are preferentially formed in the last step of the synthesis.

171. 172. 173. 174. 175. 176. 177. 178. 179. 180. 181. 182. 183. 184. 185. 186. 187. 188. 189. 190. 191. 192. 193. 194. A. Unciti-Broceta et al. Kish PE, Tsume Y, Kijek P, Lanigan TM, Hilfinger JM, Roessler BJ (2007) Mol Pharm 4:95 Byk G, Frederic M, Scherman D (1997) Tetrahedron Lett 38:3219 Byk G, Soto J, Mattler C, Frederic M, Scherman D (1998) Biotechnol Bioeng 61:81 Oliver M, Jorgensen MR, Miller A (2004) Tetrahedron Lett 45:3105 Lenssen K, Jantscheff P, Von Kiedrowski G, Massing U (2002) Chembiochem 3:852, Combinatorial synthesis of new cationic lipids and high-throughput Yingyongnarongkul BE, Howarth M, Elliott T, Bradley M (2004) Chemistry Eur J 10:463 Yingyongnarongkul BE, Radchatawedchakoon W, Krajarng A, Watanapokasin R, Suksamrarn A (2009) Bioorg Med Chem 17:176 Fara MA, Diaz-Mochon JJ, Bradley M (2006) Tetrahedron Lett 47:1011 Unciti-Broceta A, Diezmann F, Ou-Yang CY, Fara MA, Bradley M (2009) Bioorg Med Chem 17:959 Lebreton S, Newcombe N, Bradley M (2002) Tetrahedron Lett 43:2479 Fre´chet JMJ, Tomalia DA (eds) (2001) Dendrimers and other dendritic polymers.

2 41 Peptide and Peptoid Dendrimers The first polypeptide dendrimer synthesised on solid-phase was used as part of a multiple antigen peptide presentation system [222], and since this time researchers have synthesised this kind of dendrimer for a number of applications including transfection [223, 224]. The original solid-phase synthesis of an oligolysine dendrimer was based on a Boc-based synthetic strategy, starting from a b-alanyloxymethyl functionalised aminomethyl polystyrene resin. Dendrimer growth consisted of a two-step procedure: (1) coupling of pre-activated Boc-protected aminoacids and (2) subsequent acid-mediated deprotection [222], with final cleavage from the resin carried out using acetic anhydride and then a strong acid (HF).

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