Microbial Production of l-Amino Acids by Masato Ikeda (auth.), Dr. Robert Faurie, Dr. Jügen Thommel,

By Masato Ikeda (auth.), Dr. Robert Faurie, Dr. Jügen Thommel, B. Bathe, V. G. Debabov, S. Huebner, M. Ikeda, E. Kimura, A. Marx, B. Möckel, U. Mueller, W. Pfefferle (eds.)

This quantity bargains with "Microbial construction of L-Amino Acids" and offers 5 complete, specialist and real overview articles at the smooth construction of Amino Acids via program of biotechnologically optimized microorganisms. This comprises not just the fashionable recommendations of enzyme, metabolic and delivery engineering but in addition subtle analytical tools like metabolic flux research and next pathway modeling. A common evaluation approximately business strategies of Amino Acid construction offers a entire assessment approximately fresh pressure improvement in addition to fermentation applied sciences. It was once our particular curiosity to concentration the opposite articles at the most vital and most sensible promoting amino acids at the global industry i.e. L-Glutamate, L-Lysine and L-Threonine. The authors of this specific quantity have contributed considerably to the growth of Amino Acid biotechnology within the final many years and earn our exact gratitude and admiration for his or her professional evaluate articles.

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Ikeda M, Katsumata R (1995) Biosci Biotechnol Biochem 59:1600 89. Camakaris H, Cowan P, James P (1997) Eur Patent 789 073 A2 90. Azuma S, Tsunekawa H, Okabe M, Okamoto R, Aiba S (1993) Appl Microbiol Biotechnol 39:471 91. Chan E-C, Tsai H-L, Chen S-L, Mou D-G (1993) Appl Microbiol Biotechnol 40:301 92. Berry A (1996) Tibtech 14:250 93. Kurahashi O, Noda-Watanabe M, Toride Y, Takenouchi T, Akashi K, Morinaga Y, Enei H (1987) Agric Biol Chem 51:1791 94. Yajima Y, Sakimoto K, Takahashi K (1990) Japan Patent 2 190 182 A 95.

38 1 Introduction 2 Cloning of the Genes in Relation to l-Glutamate Biosynthesis 3 Analysis of Biosynthetic Pathways in Coryneform Bacteria . . 7 Sugar Metabolism . . . . . . . . . . Glycolysis and the Pentose Phosphate Pathway . . Anaplerotic Pathways . . . . . . . . . Tricarboxylic Acid (TCA) Cycle and Glyoxylate Bypath Ammonia Assimilation . . . . . . . . Energetics . . . . . . . . . . . . Transport . . . . . . . . . . . . 4 The Importance of Flux Changes in Triggering l-Glutamate Overproduction by Coryneform Bacteria .

Amplification of the glyoxylate cycle operon 153 Liao (Texas A&M University System) Amplification of the phosphoenolpyruvate synthase gene 154 Sprenger et al. (Holland Sweetener Co. VOF and Forschungszentrum Jülich GMBH) Amplification of the transaldolase gene 155 Sprenger et al. (Holland SweetenerCo. VOF and Forschungszentrum Jülich GMBH) Amplification of the sugar-phosphorylating kinase gene, inactivation of phosphoenolpyruvate-dependent glucose phosphotransferase system 156 24 M. Ikeda 3. Elimination of the PTS followed by increased glucokinase activity to phosphorylate glucose with ATP instead of phosphoenolpyruvate 4.

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