Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols by Elizabeth L. Palavecino (auth.), Yinduo Ji (eds.)

By Elizabeth L. Palavecino (auth.), Yinduo Ji (eds.)

Presenting the main updated concepts for the detection, genotyping, and research of methicillin-resistant S. aureus (MRSA), this moment variation of Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols collects chapters that make the most of the ability of whole genomic sequences and complex high-throughput applied sciences that experience driven this box to its current nation. those allow the advance of particular and quick analysis tools, the research and elucidation of mechanisms of bacterial evolution to antibiotic resistance and pathogenicity, and the identity of novel ambitions to enhance stronger healing and/or preventive brokers. Written for the Methods in Molecular Biology sequence, various chapters contain introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, without problems reproducible laboratory protocols, and tips about troubleshooting and keeping off identified pitfalls.

Authoritative and completely up-to-date, Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols, moment Edition serves as a key reference for researchers trying to organize a brand new technique to examine MRSA or perhaps for technicians and scientists engaged on different pathogens.

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Aureus population structure as most S. aureus molecular epidemiology studies were carried out in the USA, Australia (both discussed above), and Europe. In general, it is considered that Europe has a lesser burden of CA-MRSA than the USA with perhaps the exception of Greece [83]. A variety of international S. aureus strains are present, which mainly include ST80, ST1, ST8, ST30, and ST59 on the continent as well as ST93 in England. In addition, sporadic ST152 MRSA isolates have been recovered in Central Europe, the Balkan, Switzerland and Denmark and it has also been speculated that these may have derived from African ST152 MSSA strains [84].

Howden BP, Davies JK, Johnson PDR et al (2010) Reduced vancomycin susceptibility in Staphylococcus aureus, including vancomyicnintermediate strains: resistance mechanisms, laboratory detection, and clinical implications. Clin Microbiol Rev 23:99–139 92. Hsu DI, Hidayat LK, Quist R et al (2008) Comparison of method-specific vancomycin minimum inhibitory concentration values and their predictability for treatment outcome of methicillin-resistant Staphylococcus aureus (MRSA) infections. Int J Antimicrob Agents 32:378–385 93.

Observational research has also recognized the household as a potentially important transmission setting for S. aureus. Several reports document the spread of CA-MRSA within households and the potential for these strains to “ping pong” and cause recurrent infections among family members [29]. Close personal contact with household members who have a skin infection may also increase the risk of transmission and young children appear to be particularly important as reservoirs and potential vectors for CA-MRSA [30, 31].

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