Laboratory animal anaesthesia: a practical introduction for by P A Flecknell

By P A Flecknell

Using secure and powerful anaesthetic innovations may have a big impact either at the welfare of laboratory animals and the standard of the learn effects acquired in utilizing them. In occasions of justified public and medical matters over such concerns, the necessity for transparent and concise recommend on stable process is of paramount significance. although, a lot anaesthetic paintings within the laboratory is performed by means of research Read more...

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Additional info for Laboratory animal anaesthesia: a practical introduction for research workers and technicians

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Of particular concern to some research workers is the fate of inhalation anaesthetics once absorbed into the animal. A common misconception is that all of the agent which is inhaled is exhaled from the body. Many inhalation anaesthetics undergo significant metabolism, and this can result in induction of liver enzyme systems, as may occur following use of injectable anaesthetics. This can be of significance if the animal is to be used subsequently in a study which involves assessing the effects in vivo of a novel pharmaceutical or other compound.

18). "^^^^ Fig. 15. Circle system. Gas flow is made unidirectional by one way valves at (a). Carbon dioxide is removed by the soda lime cannister (b). The circuit should be connected to the animal by means of an endotracheal tube or close-fitting face-mask (c). Surplus anaesthetic gases can be scavenged from the pop-off valve (d). The disposable circuit illustrated uses an out-of-circle calibrated vaporizer to supply fresh anaesthetic gases at (e). bag. Respiratory movements result in gas being breathed out over the soda lime and into the reservoir bag, then back over the soda lime and into the animal's lungs.

If intubation of a particular species is planned, a careful examination of the pharynx and larynx should first be carried out on a post-mortem specimen. This will enable an appreciation of the anatomical relationships wfthia this area, particularly that of the soft palate and epiglottis. Once the normal anatomy of the region has been reviewed, a suitably sized endotracheal tube should be prepared. Most commercially available tubes are too long; their length should be reduced so that it approximates to the distance from the external nares to just anterior to the thoracic inlet.

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