Imaging Cellular and Molecular Biological Functions by Spencer L. Shorte (Editor), Friedrich Frischknecht (Editor)

By Spencer L. Shorte (Editor), Friedrich Frischknecht (Editor)

Imaging mobile and Molecular organic functionality offers a different number of essays by means of prime specialists, aiming at scientist and pupil alike who're drawn to all elements of contemporary imaging, from its software and up-scaling to its improvement. certainly the philosophy of this quantity is to supply pupil, researcher, PI, specialist or provost the capacity to go into this purposes box with self assurance, and to build the ability to respond to their very own particular questions.

Show description

Read Online or Download Imaging Cellular and Molecular Biological Functions (Principles and Practice) PDF

Best biophysics books

The Nile: Origin, environments, limnology and human use (Monographiae Biologicae)

What have we learnt in regards to the Nile because the mid-1970s, the instant while Julian Rzóska determined that the time had come to post a accomplished quantity in regards to the biology, and the geological and cultural historical past of that fab river? And what adjustments have in the meantime happened within the basin? The human popu- tion has greater than doubled, specifically in Egypt, but additionally in East Africa.

Erythropoietins and Erythropoiesis: Molecular, Cellular, Preclinical, and Clinical Biology (Milestones in Drug Therapy)

A entire one-source consultant to the most up-tp-date details on pink blood mobile formation and the motion of recombinant human erythropoietins. themes coated comprise: erythropoiesis, recombinant protein discovery and construction, and remedy of sufferers with anemia. the most recent theories in erythropoiesis (receptors, signaling), production, new formulations, and scientific study are mentioned.

Extra info for Imaging Cellular and Molecular Biological Functions (Principles and Practice)

Example text

The radius of the disc is determined by the NA; thus, the resolving power of an objective lens can be evaluated by measuring the size of the Airy disc. The image of the diffraction pattern can be represented as an intensity distribution as shown in Fig. 10. The bright central portion of the Airy disc and concentric rings of light correspond to intensity peaks in the distribution. In a perfect lens with no spherical aberration the diffraction pattern at the paraxial (perfect) focal point is both symmetrical and periodic in the lateral and axial planes.

The widely popular phase-contrast technique (as illustrated in Fig.

The absorption of photons is a quantum mechanical event and thus the number of photons absorbed varies according to a Poisson distribution. The accuracy of determinations of the number of photons absorbed by a particular pixel is fundamentally restrained by this inherent statistical error. This uncertainty is referred to as Poisson, statistical, or shot noise and is given by the square root of the signal or average number of photoelectrons detected. In a low-light fluorescence application the mean value of the brightest pixels might be as low as 16 photons.

Download PDF sample

Rated 4.43 of 5 – based on 29 votes