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Head-column field-amplified sample stacking in binary system capillary electrophoresis: a robust approach providing over 1000-fold sensitivity enhancement. Anal Chem 1996; 68:2523. Wey AB, Thormann W. Head-column field-amplified sample stacking in binarysystem capillary electrophoresis. The need for the water plug. Chromatographia 1999; 49(Suppl. 1):S12. 42 Weinberger 52. Weinberger R, Albin M. Quantitative micellar electrokinetic capillary chromatography: linear dynamic range. J Liq Chromatogr 1991; 14:953.
If the internal standard elutes at the correct migration time FACING PAGE FIGURE 7 Impact of added acid on peptides using field-amplified electrokinetic injection. 92 µg/mL. 36 Weinberger FIGURE 8 Effect of solute concentration on electromigration dispersion. 5 psi vacuum; detection, UV, 230 nm; solute: naproxen. (Reprinted with permission from Ref. ) Methods Development for Capillary Electrophoresis 37 with the proper peak area, we have that warm feeling that the separation is under control. Internal standards are not required for impurity determinations by area%.
15. 16. Weinberger R. Practical Capillary Electrophoresis. 2nd ed. Boston: Academic Press, 2000:462. Li T, Kennedy RT. Laser-induced fluorescence detection in microcolumn separations. Trends Anal Chem 1998; 17:484. Jandik P, Jones WR, Weston A, Brown PR. Electrophoretic capillary ion analysis: origins, principles, and applications. LC-GC 1991; 9:634. Lauer HH, McManigill D. Capillary zone electrophoresis of proteins in untreated fused silica tubing. Anal Chem 1986; 58:166. Riekkola M-L, Jussila M, Possas SP, Valko IE.