Bioorganic Chemistry Frontiers by Koji Kano (auth.), Professor Hermann Dugas, Professor Dr.

By Koji Kano (auth.), Professor Hermann Dugas, Professor Dr. Franz P. Schmidtchen (eds.)

1. ok. Kano: Selectivities of utilized Chemistry 2. A. Pl}ckthun: Antibody Engineering to review Protein-Ligand Interactions and Catalysis: The Phosphorylcholine Binding Antibodies three. M.W. Hosseini: Supramolecular Catalysis of Phosphoryl move approaches four. G. von Kiedrowski: minimum Replicator concept II: Parabolic as opposed to ExponentialGrowth five. A. Bacher, W. Eisenreich, okay. Kis, R. Ladenstein, G. Richter, J. Scheuring, S. Weinkauf: Biosynthesis of Flavins 6. C.L. Hannon, E.V.Anslyn: The Guanidinium crew: Its organic position and artificial Analogs

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2a-i!. Antigen binding fragments of an antibody that have been functionally expressed in E. coli and used as a model to study antigen binding with protein engineering. For details, see Pliickthun (1991a). (a) Fab fragment. The disulfide structure of a mouse IgA is shown, such as McPC603 discussed in the text. Note that the two chains are not covalently bound. (b) Fy fragment. (c) Fy fragment covalently linked by an engineered disulfide bond, for details see Glockshuber et al. (1990a). (d) Single-chain Fy fragment, with a genetically encoded peptide linker linking VH to VL .

Their aligned sequences are shown in Fig. 10. As the antibody TIS contains the unaltered genomic VH sequence, it has given the name to the gene, and the whole family. g. , 1987). The VH gene used by BALBjc mice is called V1 or VT1S' Mice with alterations in this gene respond poorly to PC (Hilbert and Cancro, 1988). , 1987). Of the 12 D elements known (Fig. 11), D FL16 . 1 is predominantly used, although 1 The "PC" in the antibody names signifies plasmacytoma or plasma cell tumor (not phosphorylcholine), and mineral oil ("MO") or 2,6,10,14-tetramethyl-pentadecane (pristane) ("TE") as the method of myeloma induction.

1992). The 2 to 8 repeating units appear to be identical as those of the cell-wall polysaccharide (Fig. 8), except that the third ring also carries a PC group, like on the fourth esterified to C6. This lipoteichoic acid is the pneumococcal Forssman antigen (which elicits antibodies in rabbits cross-reacting with sheep erythrocytes), but it is not the PC moiety, which is responsible. Phosphorylcholine thus occurs esterified to sugar residues in a regular array on the surface of the microbial cell.

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