Biomaterials Science and Engineering by I. Pignatello

By I. Pignatello

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3 Enzymatic modification DNA is susceptible to modification including cleavage and chemical deletion or addition by a large number of enzymes. Nature has created a rather delicate and precise machinery to manipulate, such as cutting, ligating, unwinding, folding, synthesizing, initiating, modifying, and deleting, DNA in vivo(Braun & Keren 2004, Watson et al. 2003). Many of the enzymes involved in the process have been identified, namely, restriction enzymes, ligase, helicase, gyrase, polymerase, primase, proofreading exonuclease, and a host of other enzymes.

1999). Functionalized silica synthesized by sol–gel process. J Non-Cryst Solids, Vol. 247, No 1-3, pp. 124-128. , Bohn, E. (1968). Controlled growth of monodisperse silica spheres in the micron size range. J. Coll. Inter. , Vol. 26, No. 1, pp. 62-69. 35 Papacídero, A. , Rocha, L. , Caetano, B. , Molina, E. , Sacco, H. , Nassar, E. , Ciuffi, K. J. (2006). Preparation and characterization of spherical silica–porphyrin catalysts obtained by the sol–gel methodology. Coll and Surfaces, Vol. 275, No.

The calcium atoms are distributed over the IC1 matrix, but they appear as some clusters that coincide with the fluoride clusters, thereby indicating the presence of CaF2 crystals, as shown by XRD. Biomaterials and Sol-Gel Process: A Methodology for the Preparation of Functional Materials 19 Fig. 19. Elemental distribution mapping of the material IC1 treated at 110 oC. For the material IC2, the MEV/EDS analysis (Figure 20) revealed that the silicon atoms are located in the same regions as the aluminum and oxygen atoms, pointing to the possible formation of aluminosilicate.

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