Biochemical and Biotechnological Applications of by A. Peter Snyder (Eds.)

By A. Peter Snyder (Eds.)

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Additional resources for Biochemical and Biotechnological Applications of Electrospray Ionization Mass Spectrometry

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The pneumatically assisted ESI evolved from a combination of earlier pure electrospray devices (17) and ion evaporation devices (18) and thus the term "Ion Spray" was coined (19). By mechanically shearing the liquid at the sprayer tip, pneumatic nebulizers have proven to effectively generate small droplets from high flows and high aqueous solutions without adjustment to account for changing mobile phase condition. Over the years pneumatic nebulization has proven to be the most simple and practical approach to solving this problem.

Ch002 Thomas Covey Perkin Elmer-Sciex, 71 Four Valley Drive, Concord, Ontario L4K 4V8, Canada Electrospray mass spectrometry has gained prominence as the most versatile detector for high performance liquid chromatography and capillary electrophoresis. Key issues of primary concern when considering this technique for any particular application are sensitivity, dynamic range, mobile phase flow rates and composition, appropriate HPLC columns and pumps, stream splitting, concentration sensitivity, and types of molecules amenable to ionization.

The tendency of PAH compounds to ionize by chemical ionization has been demonstrated earlier and it is suggested that atmospheric pressure chemical ionization is the method of choice for the analysis of complex PAH samples (31). + 4. Inorganic anions and cations. Some of the earliest discussions of applications of ESI showed data on elemental species (32). This area has only been sparsely exploited to date but interest is rapidly climbing, especially for purposes of elemental speciation (33-35).

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